論文和摘要
Summary: Key to the delivery of preventive healthcare is the clinical adoption of novel assays that enable early disease detection. This review article discusses the clinical utility and validation of miRNA-based molecular diagnostics for early disease detection through large-cohort studies and key considerations for developing multi-analyte clinical assays.
Summary: This paper describes the largest comprehensive, multi-center study to date involving diverse ethnic groups, for the identification of circulating miRNAs for breast cancer screening. The panel detects breast cancer in Caucasian and Asian populations, including pre-malignant lesions (stage 0) and early-stage (stages 1-2) cancers.
Summary: Mammography is extensively used for breast cancer screening but has high false-positive rates. This paper describes the discovery, development, and validation of a serum 6-miRNA panel to discriminate between malignant and benign breast lesions among women with abnormal mammograms.
Summary: Discovery, development, and large-scale prospective clinical validation of GASTROClear – a serum 12-miRNA biomarker panel for early detection of gastric cancer. The panel was validated to detect 87% of all gastric cancer patients, including 87.5% of Stage 1 tumours.
Summary: Discovery, development, and validation of a serum 5-miRNA biomarker panel for early detection of non-small call lung cancer (NSCLC). The panel was validated to detect 81.3% of all NSCLC patients, including 83.0% of Stage 1 tumours.
Summary: Paper describing the development of a high-performance real-time RT-PCR assay that allows specific and rapid quantification of existing and novel miRNAs using a fast thermocycling profile. The assay had a wide dynamic range (more than 7 orders of magnitude) and was capable of detecting miRNAs from as little as 1 picogram of total RNA (or as few as 10 cells).
Author(s): Choi H, Koh HWL, Zhou L, Cheng H, Loh TP, Parvaresh Rizi E, Toh SA, Ronnett GV, Huang BE, Khoo CM. Frontiers in Physiology 2019 Apr 5;10:379
Author(s): Wong LL, Zou R, Zhou L, Lim JY, Phua DCY, Liu C, Chong JPC, Ng JYX, Liew OW, Chan SP, Chen YT, Chan MMY, Yeo PSD, Ng TP, Ling LH, Sim D, Leong KTG, Ong HY, Jaufeerally F, Wong R, Chai P, Low AF, Lund M, Devlin G, Troughton R, Cameron VA, Doughty RN, Lam CSP, Too HP, Richards AM. Journal of the American College of Cardiology 2019 Mar 26;73(11):1300-1313
