Spatial Transcriptomics powered by Stereo-seq (SpaTial Enhanced REsolution Omics-sequencing) offers nanoscale subcellular resolution with a large field of view for spatial transcriptomics sequencing. It is a promising technology for research in spatial characterization of the tumor microenvironment, construction of 3D transcriptomics model, spatiotemporal transcriptomics atlases in mammalian developmental biology, and many more.
MiRXES Genomics offers an end-to-end service for Stereo-seq workflow, from tissue sectioning to bioinformatics tertiary analysis. Gene expression data is integrated with spatial information at a nanoscale resolution to generate unprecedented details.
Service | Expected Data Quantity | Sequencing Platforms |
|---|---|---|
Stereo-seq | 1.5 Billion reads per chip, Chip size of 1cm x 1cm | DNBSEQ-T7 DNBSEQ-G400 |
Service | Suggested Sequencing Depths | Sequencing Platforms |
|---|---|---|
Human Whole Genome Sequencing | Rare Diseases: 30x (90Gb)
Oncology: ≥ 50x (≥ 150Gb) | DNBSEQ-T7
DNBSEQ-T10 |
Service | Suggested Sequencing Depths | Sequencing Platforms |
|---|---|---|
Human Whole Exome Sequencing | Rare Diseases: 100x (10Gb)
Oncology: ≥ 200x (≥ 20Gb) | DNBSEQ-T7
|
RNA sequencing (RNASeq) is an indispensable tool for transcriptome-wide analysis of differential gene expression and structural analysis of RNAs.
MiRXES Genomics offers an efficient package for rRNA depletion with synthesis of cDNA libraries from a wide input range of total RNA. These libraries are then sequenced on using DNBseq technology, generating high quality data for comprehensive analysis of gene expression analysis and discovery of alternate splicing events, gene fusions and other transcriptome aberrations.
Service | Suggested Sequencing Depths | Sequencing Platforms |
|---|---|---|
Human RNA Sequencing | ≥ 40M reads (≥ 12Gb)
≥ 60M reads (≥ 18Gb) | DNBSEQ-T7
|
Human whole genome methylation sequencing (hWGMS) offers the ability to interrogate methylation status at a single base resolution epigenome-wide. It has become an increasingly accessible technique to investigate changes in methylation patterns associated with complex diseases including cancer and neurological disorders.
Equipped with the largest sequencers in the world, MiRXES Genomics enables high-throughput methylation analysis by revolutionizing the cost of sequencing and providing end-to-end bioinformatics support for large datasets.
Service | Suggested Sequencing Depths | Sequencing Platforms |
|---|---|---|
Human Whole Genome Methylation Sequencing | Basic analysis: 30x Deep analysis: ≥ 100x | DNBSEQ-T7 DNBSEQ-T10 |
Platforms | Sequencing Read Lengths | Purchase UOM |
|---|---|---|
MGI T7 | PE100, PE150 | Flow cells/Full run |
MGI T10 | PE100, PE150 | Flow cells/Full run |
Illumina NovaSeq 6000 | PE150 | Lanes/Flow cells |
