our microrna technology
Unique RT Primer
Conformation-restricted microRNA-specific RT primer. Efficiently hybridizes to mature, but not precursor form, of target microRNA.
Specific Real-Time PCR Primers
microRNA-specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification.
Tailored RT-qPCR Reagents
Optimized RT and qPCR master mixes that enhance signal-to-noise ratio.
MiRXES ID3EAL™ microRNA qPCR assays yielded highly reproducible profiling results for more than 200 microRNAs from 30 cancer patient sera over a year in two independent laboratories (Pearson correlation > 0.95). The ease of use enables even first time users to generate consistent results from technical and biological replicates.
The combination of microRNA-specific RT primer and nested qPCR primer pairs enable our assays to efficiently discriminate between highly homologous microRNA family members, even those with single nucleotide differences.