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Conformation-restricted microRNA-specific RT primer. Efficiently hybridizes to mature, but not precursor form, of target microRNA.
microRNA-specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification.
Optimized RT and qPCR master mixes that enhance signal-to-noise ratio.
Increased Sensitivity
Optimized RT-qPCR primers and reagents ensure efficient target amplification from limited amounts (~1 pg) of input RNA.
Increased Specificity
No universal primers. Every assay utilizes three microRNA-specific primers to discriminate between microRNAs with single nucleotide differences.
Speedy
Detection
From RNA to Ct result in less than 2 hours.
Reliable
Data
Assays optimized by our proprietary algorithm and experimentally validated.
Convenience
Provided in a complete kit to minimize set-up time. Compatible with all major qPCR instruments.
Superior Sensitivity
The unique three primer design of the ID3EAL™ miRNA qPCR Assays yields class leading sensitivity compared to other miRNA detection systems. These assays are shown to have more consistent performance over miRNAs with varying sequences especially those with high AT content.
Greater Reproducibility
MiRXES ID3EAL™ microRNA qPCR assays yielded highly reproducible profiling results for more than 200 microRNAs from 30 cancer patient sera over a year in two independent laboratories (Pearson correlation > 0.95). The ease of use enables even first time users to generate consistent results from technical and biological replicates.
Unparalleled Specificity
The combination of microRNA-specific RT primer and nested qPCR primer pairs enable our assays to efficiently discriminate between highly homologous microRNA family members, even those with single nucleotide differences.