Platform Technology | MiRXES | microRNA discovery and diagnostics

Class-leading microRNA RT-qPCR Technology

MiRXES’ patented reverse transcription (RT)-quantitative polymerase chain reaction (qPCR) technology platform enables sensitive, specific, and robust detection of microRNAs and other non-coding RNAs in biofluids and tissue samples.

This platform has been independently validated to be more sensitive than other RT-qPCR technologies for microRNA quantification.

Increased Sensitivity

Optimized RT-qPCR primers and reagents ensure efficient target amplification from limited amounts (~1 pg) of input RNA.

Increased Specificity

No universal primers. Every assay utilizes three microRNA-specific primers to discriminate between microRNAs with single nucleotide differences.

Speedy
Detection

From RNA to Ct result in less than 2 hours.

Reliable
Data

Assays optimized by our proprietary algorithm and experimentally validated.

Convenient

Provided in a complete kit to minimize set-up time. Compatible with all major qPCR instruments.

Key Features

Conformation-restricted microRNA-specific RT primer. Efficiently hybridizes to mature, but not precursor form, of target microRNA.

microRNA-specific forward and reverse real-time PCR primers confer further specificity and enable robust amplification.

Optimized RT and qPCR master mixes that enhance signal-to-noise ratio.

Superior Sensitivity

The unique three primer design of our assays yields class-leading sensitivity compared to other miRNA detection systems.

These assays were shown to have more consistent performance across miRNAs with varying sequences especially those with high AT content.

Greater Reproducibility

Our assays yielded highly reproducible profiling results for more than 200 microRNAs from 30 cancer patient serum samples over a year in two independent laboratories (Pearson correlation > 0.95).

This ease of use enables even first time users to generate consistent results from technical and biological replicates.

Unparalleled Specificity

The combination of microRNA-specific RT primer and nested qPCR primer pairs enable our assays to efficiently discriminate between highly homologous microRNA family members, even those with single nucleotide differences.