Publicaciones y resúmenes

Key Scientific Publications

Advances in quantifying circulatory microRNA for early disease detection

Author(s): Cheong JK, Tang YC, Zhou LH, Cheng H, and Too HP. Current Opinion in Biotechnology 2022 Jan 6; 74:256–262


Summary: Key to the delivery of preventive healthcare is the clinical adoption of novel assays that enable early disease detection. This review article discusses the clinical utility and validation of miRNA-based molecular diagnostics for early disease detection through large-cohort studies and key considerations for developing multi-analyte clinical assays.

Development and validation of a circulating microRNA panel for the early detection of breast cancer

Author(s): Zou R, Loke SY, Tang YC, Too HP, Zhou L, Lee ASG, and Hartman M. British Journal of Cancer 2022 Jan 10


Summary: This paper describes the largest comprehensive, multi-center study to date involving diverse ethnic groups, for the identification of circulating miRNAs for breast cancer screening. The panel detects breast cancer in Caucasian and Asian populations, including pre-malignant lesions (stage 0) and early-stage (stages 1-2) cancers.

Development of a microRNA panel for classification of abnormal mammograms for breast cancer

Author(s): Zou R, Loke SY, Tan VKM, Quek ST, Jagmohan P, Tang YC, Madhukumar P, Tan BKT, Yong WS, Sim Y, Lim SZ, Png E, Lee SYS, Chan MYP, Ho TSJ, Khoo BKJ, Wong SLJ, Thng CH, Chong BK, Teo YY, Too HP, Hartman M, Tan NC, Tan EY, Lee SC, Zhou L, and Lee ASG. Cancers 2021 Apr 28;13(9):2130


Summary: Mammography is extensively used for breast cancer screening but has high false-positive rates. This paper describes the discovery, development, and validation of a serum 6-miRNA panel to discriminate between malignant and benign breast lesions among women with abnormal mammograms. 

Development and validation of a serum miRNA biomarker panel for detecting gastric cancer in a high-risk population

Author(s): So JBY, Kapoor R, Zhu F, Koh C, Zhou L, Zou R, Tang YC, Goo PCK, Rha SY, Chung HC, Yoong J, Yap CT, Rao J, Chia CK, Tsao S, Shabbir A, Lee J, Lam K-P, Hartman M, Yong WP, Too HP, Yeoh KG. Gut 2021 May; 70(5):829-837. Epub 2020 Oct 7.


Summary: Discovery, development, and large-scale prospective clinical validation of GASTROClear – a serum 12-miRNA biomarker panel for early detection of gastric cancer. The panel was validated to detect 87% of all gastric cancer patients, including 87.5% of Stage 1 tumours.

Development of a serum miRNA panel for detection of early stage non-small cell lung cancer

Author(s): Ying L, Du L, Zou R, Shi L, Zhang N, Jin J, Xu C, Zhang F, Zhu C, Wu J, Chen K, Huang M, Wu Y, Zhang Y, Zheng W, Pan X, Chen B, Lin A, Tam JKC, van Dam RM, Lai DTM, Chia KS, Zhou L, Too HP, Yu H, Mao W, Su D. Proceedings of the National Academy of Sciences of the United States of America (PNAS) 2020 Oct 6;117(40):25036-25042


Summary: Discovery, development, and validation of a serum 5-miRNA biomarker panel for early detection of non-small call lung cancer (NSCLC). The panel was validated to detect 81.3% of all NSCLC patients, including 83.0% of Stage 1 tumours.

High-performance quantification of mature microRNAs by real-time RT-PCR using deoxyuridine-incorporated oligonucleotides and hemi-nested primers

Author(s): Wan G, Lim QE, Too HP. RNA 2010 Jul;16(7):1436-45.


Summary: Paper describing the development of a high-performance real-time RT-PCR assay that allows specific and rapid quantification of existing and novel miRNAs using a fast thermocycling profile. The assay had a wide dynamic range (more than 7 orders of magnitude) and was capable of detecting miRNAs from as little as 1 picogram of total RNA (or as few as 10 cells).

Other Scientific Journal Papers

Multiomics analyses of cytokines, genes, miRNA, and regulatory networks in human mesenchymal stem cells expanded in stirred microcarrier-spinner cultures

Author(s): Lam ATL, Lee AP, Jayaraman P, Tan KY, Raghothaman D, Lim HL, Cheng H, Zhou L, Tan AHM, Reuveny S, Oh S. Stem Cells Research 2021 Feb 26;53:102272.

Molecular exploration of paediatric intracranial germinomas from multi-ethnic Singapore

Author(s): Low SYY, Cheng H, Zou R, Ng LP, Kuick CH, Sulaiman NBS, Chang KTE, Low DCY, Zhou L, Seow WT. BMC Neurology 2020; 20:415

Evaluating the use of microRNA blood tests for gastric cancer screening in a stratified population-level screening program: an early model-based cost-effectiveness analysis

Author(s): Kapoor R, So JBY, Zhu F, Too HP, Yeoh KG, Yoong JSY. Value in Health 2020 Sep;23(9):1171-1179.

Plasma protein and microRNA biomarkers of insulin resistance: a network-based integrative -omics analysis

Author(s): Choi H, Koh HWL, Zhou L, Cheng H, Loh TP, Parvaresh Rizi E, Toh SA, Ronnett GV, Huang BE, Khoo CM. Frontiers in Physiology 2019 Apr 5;10:379
Combining circulating microRNA and NT-proBNP to detect and categorize heart failure subtypes
Author(s): Wong LL, Zou R, Zhou L, Lim JY, Phua DCY, Liu C, Chong JPC, Ng JYX, Liew OW, Chan SP, Chen YT, Chan MMY, Yeo PSD, Ng TP, Ling LH, Sim D, Leong KTG, Ong HY, Jaufeerally F, Wong R, Chai P, Low AF, Lund M, Devlin G, Troughton R, Cameron VA, Doughty RN, Lam CSP, Too HP, Richards AM. Journal of the American College of Cardiology 2019 Mar 26;73(11):1300-1313

Trehalose significantly enhances the recovery of serum and serum exosomal miRNA from a paper-based matrix

Author(s): Neo SH, Chung KY, Quek JM, Too HP. Scientific Reports 2017 Nov 30;7(1):16686.

Establishing multiple omics baselines for three Southeast Asian populations in the Singapore Integrative Omics Study

Author(s): Saw WY, Tantoso E, Begum H, Zhou L, Zou R, Cheng H, Chan SL, Tan LW, Wong LP, Xu W, Moong DKN, Lim Y, Li B, Pillai NE, Peterson TA, Bielawny T, Meikle PJ, Mundra PA, Lim WY, Luo M, Chia KS, Ong RT, Brunham LR, Khor CC, Too HP, Soong R, Wenk MR, Little P, Teo YY. Nature Communications 2017 Sep 21;8(1):653.

Nano-topology guided neurite outgrowth in PC12 cells is mediated by miRNAs

Author(s): Cheng H, Zhou L, Li B, Zhu M, Too HP, Choi WK. Nanomedicine 2014 Nov;10(8):1871-5.

MicroRNA as potential modulators in chemoresistant high-grade gliomas

Author(s): Low SY, Ho YK, Too HP, Yap CT, Ng WH. Journal of Clinical Neuroscience 2014 Mar;21(3):395-400.

Direct quantification of mRNA and miRNA from cell lysates using reverse transcription real time PCR: a multidimensional analysis of the performance of reagents and workflows

Author(s): Ho YK, Xu WT, Too HP. PLoS One 2013 Sep 5;8(9):e72463.

Glial cell-line derived neurotrophic factor and neurturin regulate the expressions of distinct miRNA precursors through the activation of GFRalpha2

Author(s): Yoong LF, Wan G, Too HP. Journal of Neurochemistry, August 2006

Scientific Conference Presentations/Posters

Feasibility of microRNA signatures for detection of early stage pulmonary hypertension

Author(s): Waxman A, Fong Y, Jatkoe T, Cheng H, Zhou L, Bridges C. European Respiratory Journal 2020 56:1532 (European Respiratory Society International Congress, September 2020)

Feasibility of microRNA-based signatures for early detection of pulmonary hypertension
using machine learning methods

Author(s): Lawrie A, Toshner M, Fong Y, Jatkoe T, Cheng H, Zhou L, Bridges C, Howard LS, Wilkins MR. American Journal of Respiratory and Critical Care Medicine 2020;201:A6352 (American Thoracic Society International Conference, May 2020)

Detection of gastric cancer using a serum microRNA assay

Author(s): So BYJ, Zhou L, Zou R, Zhu F, Koh CJ, Rha SY, Chung HC, Rao J, Chia CK, Tsao S, Shabbir A, Yong WP, Too HP, Yeoh KG. United European Gastroenterology Journal Volume 7 Issue 8 (Supplement), October 2019 (United European Gastroenterology Week 2019 Meeting – Presentation)

Prospective validation of a serum miRNA panel for early detection of gastric cancer.

Author(s): Zhou L, So J, Kapoor R, Zhu F, Zou R, Koh CJ, Rha SY, Chung HC, Yoong J, Yap CT, Rao J, Chia CK, Tsao S, Shabbir A, Lam KP, Hartman M, Yong WP, Too HP, Yeoh KG. Journal of Clinical Oncology 2019 (American Society for Clinical Oncology 2019 Meeting)

Identification and validation of a serum microRNA panel for detection of early-stage breast cancer

Author(s): Zou R, Zhou L, Loke SY, Too HP, Lee A. Journal of Clinical Oncology 2019 37:suppl, 3051 (American Society for Clinical Oncology 2019 Meeting)

Development and validation of a serum miRNA biomarker panel for the detection of early stage lung cancer

Author(s): Ying L, Zou R, Du L, Shi L, Zhang N, Jin J, Wu J, Zhang F, Chen K, Yu H, Mao W, Su D. Cancer Research 2018;78(13 Suppl):4408 (American Association for Cancer Research 2018 Meeting)

A serum microRNA biomarker panel for detection of gastric cancer

Author(s): So J, Zou R, Zhou L, Too HP, Zhu F, Teo YY, Yap CT, Rha SY, Kono K, Yoong J, Yeoh KG, Yong WP. Journal of Clinical Oncology 2015 33:15_suppl, 4060-4060 (American Society for Clinical Oncology 2015 Meeting)

Circulating microRNAs as diagnostic and prognostic markers for heart failure

Author(s): Wong LL, Lim JY, Zhou L, Zou R, Chan MMY, Lam CSP, Too HP, Richards AM. Circulation 2014 130:A15048 (American Heart Association 2014 Meeting)

Identification and validation of serum microRNA biomarker for early detection of gastric cancer

Author(s): Zhou L, So JB, Zou R, Feng Z, Yong WP, Teo YY, Chia KS, Yap CT, Kono K, Too HP, Yeoh KG. Gastroenterology 2014 Volume 146, Issue 5, S-31 (American Gastroenterological Association 2014 Meeting)

REDES SOCIALES

QUIÉNES SOMOS

SOLUCIONES

TECNOLOGÍA

PROYECTO

SALA DE PRENSA

Copyright © 2024 MiRXES Pte Ltd. All rights reserved. | Privacy Policy
Scroll to Top