觅瑞的专利逆转录(RT)定量聚合酶链反应(qPCR)技术平台可对生物流体和组织样品中的miRNA和其他非编码RNA进行灵敏,特异和强大的检测。
该平台已经过独立验证,比其他RT-qPCR技术对miRNA量化更敏感。
优化的RT-qPCR引物和试剂,可确保从限量(~1 pg)输入RNA中,实现有效的目标扩增。
非通用引物。每个测定都利用三种miRNA特异性引物来区分具有单核苷酸差异的miRNA。
从RNA到Ct结果,不到2小时。
检测分析由我们的专有算法优化,并经过实验验证。
提供完整的套件,以尽量减少设置时间。兼容所有主流qPCR仪器。
构象受限的miRNA特异性逆转录引物。有效杂交到目标miRNA的成熟形式,而不是前体形式。
miRNA特异的正向和反向qPCR引物可提供进一步的特异性,并能实现可靠的扩增。
优化的RT和qPCR预混液可增强信噪比。
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